Event - HRM workshop: Best Practice in High Resolution Melting Curve Analysis

What

This Workshop will address basic principles of HRM technology and new developments, but will most of all specifically focus on its application in Genome Diagnostic Laboratories.

Background information

EuroGentest Unit 5 Industry: EuroGentest (EUGT) is an EU-funded Network of Excellence looking at different aspects of genetic testing, such as quality management, genetic test evaluation/validation and implementation of new techniques. EUGT encourages the harmonization, validation and standardization of services within Europe.
Unit 5 (Industry) is especially involved in the recruitment, validation/evaluation of new techniques that have a potential application and value in genome diagnostic testing. In 2007 we started with the evaluation of High Resolution Melting curve analysis (HRMA) and since then we have evaluated 3 platforms that perform HRMA including the LightScanner from Idaho Technologies (IT), the LightCycler 480 from Roche and the Rotor-Gene 6000 from Corbett (Qiagen). Recently, we published the results of our validation study on the lightScanner (IT) platform, "Diagnostic guidelines for high-resolution melting curve (HRM) analysis: an interlaboratory validation of BRCA1 mutation scanning using the 96-well LightScanner" in the journal Human Mutation (Hum Mutat. 2009 Jun;30(6):899-909).

HRM workshop.

This Workshop will address basic principles of HRM technology and new developments, but will most of all specifically focus on its application in Genome Diagnostic Laboratories. In this respect it will address topics such as, how to design and set up an HRM gene test including genotype analysis of frequent occurring polymorphisms, diagnostic guidelines, other technical applications, and most of all HRM data analysis and interpretation. We encourage people to highlight into in their submission form topics to be addressed in the workshop. Finally, we hope to create a good interactive environment in which we can discuss important aspects that arise when validating this technique for different applications in diagnostics.
Program details will follow soon.

Location.

The workshop will be held at the Leiden University Medical Centre (LUMC) in Leiden, the Netherlands. Further details will follow

Registration.

390 euro per person when registered and paid before August 3, 2009
The fee covers all sessions, participant's workshop manual, two lunches, refreshments, Thursday evening dinner and hotel accommodation including breakfast for September 16 and 17.

Register here.

Accommodation.

Accommodation is included for Wednesday 16 and Thursday 17 September.
Hotel : Van der Valk Hotel Leiden
Restaurant Het Haagsche Schouw
Haagse Schouwweg 14
2332 KG Leiden
[Transportation to LUMC will be arranged.]

Contact and more information.

N. van der Stoep, PhD
Leiden University Medical Center (LUMC), Center for Human and Clinical Genetics, Laboratory for Diagnostic Genome Analysis (LDGA),
Building 2, room S-06-042
Einthovenweg 20, Leiden
The Netherlands
Tel. +31 71-5269832
E-mail:

 

Payment information

390 euro per person when registered and paid before August 3, 2009

The fee covers all sessions, participant's workshop manual, two lunches, refreshments, Thursday evening dinner and hotel accommodation including breakfast for September 16 and 17.

An email with a personal payment reference will be issued to you upon receipt of this form. Your registration is NOT confirmed until full payment is received.

Please remit payment within 20 days after receipt of your invoice. Make sure you specify the correct structured personal reference code (number) mentioned in the email message send upon registration.

Without this reference code, your payment cannot be processed automatically, which can cause a delay in your submission and subsequent registration to the workshop. Therefore please wait after registration for this email message before your start payment. ( )

Bank details:

Name of the account holder:	LUMC Division 5
Address of the account holder:	Albinusdreef 2
	2333 ZA Leiden
	The Netherlands
Name of the bank:	ABN AMRO
Address of the bank:	Postbus 90
	1000 AB Amsterdam
SWIFT code:	ABN ANL 2A
IBAN code:	NL91ABNA0464156629
Account number:	46.41.56.629
Reference:	8228/50088 + reference code*

* Will be sent to you upon registration

 

Preliminary Program

Morning session on both days, 17 and 18 September will be from 9 to 12.30.

Topics will include, general introduction on HRM technology, new developments, primer design, critical features and limitations that need to be taken into account when using and setting up HRM for mutation detection and genotyping assays in diagnostics, other and new technical applications and validation studies on mutation scanning tests for the BRCA genes on three different platforms, the LightScanner (Idaho technology), the LighCycler 480 (Roche) and the Rotorgene 6000 (Corbett/Qiagen).
Guest speakers include Jason McKinney (USA), Kathleen Claes (BE), Johan den Dunnen (NL), Gaelle Benais-pont (CH), Tom Janssens (BE), Ruud van den Bogaard (NL) and Andrea Gehrig (DE) and several others.

Both afternoons will be dedicated to practical HRM data analysis including frequent polymorphism genotyping analysis, mosaic detection, and X linked disease/hemizygous mutation detection. In addition we will address primer design for HRM tests and on Friday 18 September we plan group discussion sessions to address specific problems that can be encountered when setting up, applying and validating HRM tests.
As indicated earlier there will be a dinner party on Thursday evening
Friday September 18 the official program will stop at 4 PM, and we will finish with a drink.